Prolonged activity of a recombinant manganese superoxide dismutase through a formulation of polymeric multi-layer nanoassemblies targeting cancer cells

A new isoform of human manganese superoxide dismutase (SOD) has been recently isolated and obtained in a synthetic recombinant form termed rMnSOD. As compared to other SODs, this exhibits dramatically improved cellular uptake an intense antioxidant antitumoral activity. Unfortunately, its use is severely hampered as active pharmaceutical ingredient (API) solution suffers from remarkable instability, which realizes interplay unfolding aggregation phenomena. This leads the API be ineffective after three weeks only when stored at 4°C. formulation strategy was undertaken mitigate instability. based on incorporation hyaluronic acid layer-by-layer deposition over chitosan-n-acetyl cysteine- monolayer nanoemulsion (NE) subsequent coverage with further external interface cysteine. The constructs were tested selected panel healthy cancerous cell lines. enhanced API's effect vitro already time zero, maintaining efficacy anticancer agent until up 30